OBESITY – INSULIN RESISTANCE – DIABETES PRECLINICAL CRO SERVICES

Our common goal with our clients is to tackle the worldwide obesity and type 2 diabetes epidemic. Physiogenex offers preclinical CRO services using innovative and benchmarked animal models of obesity, insulin resistance and type 2 diabetes. Along with these services, our clients take advantage of our great experience and consultancy expertise to optimize and accelerate their drug development programs.

Our clients benefit from our experts capabilities, which include classical in vivo test experiments (OGTT, ITT) and our innovative and gold standard radio-tracer based experiments: euglycemic – hyperglycemic – hyperinsulinemic clamp with 3H-glucose (for glucose turnover, hepatic glucose production, etc.) and 14C-2-deoxyglucose (glucose tissue uptake in muscle, adipose fat pads, etc.).

Our integrative approach provides mechanistic understanding of your drug candidates’ efficacy for better translation to the clinical setting.

In vivo disease models for obesity, insulin resistance and diabetes

Nutritional animal models of obesity and insulin resistance

Diet-induced obese mouse (60% high fat diet):

Original free-choice diet-induced obese rat:

Original free-choice diet-induced obese hamster

Insulin resistant and dyslipidemic hamster model:

High fat diet CETP-apoB100 mouse:

Genetic models of obesity and diabetes

Ob/ob and db/db mice:

Zucker diabetic fatty rat, Zucker fatty rat and Goto-Kakizaki rat (diabetes):

Chemical models of type 1 diabetes

Streptozotocin (STZ) rat or rat (diabetes):

Preclinical CRO Services for obesity, insulin resistance and diabetes

Ex vivo screening
  • Muscles/adipocytes ex vivo glucose uptake
  • Muscles/adipocytes ex vivo FFA oxydation
  • Adipocytes ex vivo lipolysis

 

All our ex vivo assays
In vivo drug efficacy experiments

Glucose homeostasis

  • Glucose and insulin tolerance test
  • Hyperinsulinemic euglycemic/hyperglycemic clamps with 3H-glucose (hepatic glucose production, turnover, glycolysis, etc.) and 14C-2-deoxyglucose (glucose uptake)
  • In vivo individual tissue glucose uptake

Lipid metabolism

  • Lipid tolerance test
  • Lipogenesis in vivo with 3H2O or 14C-acetate

 

in vivo studies
In vivo services
  • Body weight food/water intake, organs collection, weight and fixation (fat pads, liver, etc.), blood collection and tissue dissection
  • Cardiometabolic parameters (echocardiography)
  • Indirect calorimetry (energy expenditure), Echo MRI (body composition)

 

in vivo studies
Biochemistry assays and histology
  • Standard hormones (insulin, glucagon, adiponectin, leptin, etc.)
  • Inflammation (plasma cytokines)
  • Tissue analysis (lipids, gene and protein expression)
  • Histology, immunohistology and histopathological scoring

 

biochemical assays and histology analysis

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