BIOCHEMICAL ASSAYS AND HISTOLOGY ANALYSIS

Biochemical assays and histological analysis are complementary with in vivo / ex vivo studies to fully characterize the potential of your drug candidates and their impact on metabolic molecules and tissues in the organisms.

Physiogenex provides a large range of biochemical assays and histology analyses to evaluate your drug efficacy.

To measure the main readouts needed to demonstrate the benefits of your drugs, we offer:

  • Colorimetric assays:

Plasma and hepatic lipids (cholesterol, triglycerides, fatty acids)
Lipoproteins (direct LDL-C and HDL-C assays, lipoprotein FPLC profile, apo A-I, apoB)
Plasma ALT/AST
Total ketone bodies
Glycerol
Phospholipids
Albumin
Creatinine
Faecal total cholesterol and bile acids

  • ELISA and multiplex assays:

Hormones (insulin, glucagon, adiponectin, leptin, etc.)
Cytokines panel (IL-1b, IL-6, MCP-1, TNF-alpha, etc.)

  • Western Blot analysis (WES technology): for any protein, on any sample/tissue.
  • Microbiome, lipids, bile acids profiling
  • Gene expression by qPCR on any tissue:

Lipogenesis
Glucose metabolism
ER and oxidative stress
Inflammation
Fibrosis

  • Histology analysis:

H&E
Sirius Red
Masson Trichrome
ORO
PAS staining
Immunohistochemistry (F4/80, CD68, ED1, collagen III, alpha-SMA, etc.)
NAS scoring (steatosis, inflammation, hepatocyte ballooning, fibrosis)
Nephropathy histopathology scoring (glomerulosclerosis, interstitial fibrosis, etc.)

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