Indirect calorimetry

Gold standard method for determining the efficacy of your anti-obesity compound, by measuring the energy expenditure rate as well as the type of substrate used.

Key benefits

  • Essential and robust in vivo data for your records:
    • Indirect calorimetry provides unique insights by measuring respiratory gases in conscious free-moving mice and the animals’ activity.
    • Indirect calorimetry establishes which type of substrate (carbohydrates, lipids) is preferentially used by animals treated with your compound (mainly carbohydrates or free fatty acids).

Species

  • Mouse

Parameters evaluated   

  • O2 consumption
  • CO2 production
  • Respiratory quotient RQ
  • Energy expenditure (kcal/day/kg0.75)
  • Activity

Add-on studies

  • Free fatty acid turnover, with specific emphasis on individual tissue FFA storage rate, providing a whole-body tissue by tissue overview of your compound's efficacy on lipotoxicity.

References

  • This technique has been validated in collaboration with INSERM.
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