High Fat Diet mouse model

Muscle Adipose tissue Liver Pancreas Macrophage
A reference mouse model totally devoted to insulin resistance due to lipotoxicity

Key benefits

  • Tailor-made nutritional model to meet your objectives with a protocol tailored to obtain the required phenotype (lean diabetic, obese non diabetic or obese diabetic animals)
  • Pharmacological model relevant to the main classes of approved anti-diabetic compounds
  • Assess the efficacy of your compound in mice displaying major features of insulin resistance
  • Take advantage of Physiogenex's unique expertise with this model.

Animal Model

  • Background Strain: C57BL/6 mice
  • Gender/Weight/Age: Male mice, young mice (4-6 weeks), adult mice (~12 weeks)       
  • Diet: HFD (~78% fat %E)
  • Time on diet: Adult mice 4 weeks, young mice 12 weeks (or more on request)
  • Positive reference compounds: metformin, rosiglitazone

 

HFD 78% graph 1

Pathophysiological features and scientific data

(after 12 weeks of diet)

  • Mild obesity (mainly visceral): ~32g vs ~28g in control chow mice
  • Slight hyperglycemia in fasting state
  • Glucose intolerance
  • Insulin resistance (hepatic and peripheral)

 

HFD 78% graph 2
HFD mouse 78% graph 3
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Pharmacological relevance

Reference compound: Metformin

  • Treatment: acute

 

HFD 78% graph 5

Results :

  • improvement in whole body glucose utilization
  • increase in glycogen synthesis rate
  • demonstration of the insulin-sensitizing effect in acute conditions

Reference compound: Rosiglitazone

  • Treatment: chronic (once a day)
  • Duration: 4 weeks
  • Dose: 10 mg/kg
HFD 78% graph 6
HFD 78% graph 7
HFD 78% graph 8

Results:

  • reduction in hepatic glucose production in basal state
  • improvement in whole body glucose utilization in insulin conditions
  • increase in glucose uptake by muscle and heart and decrease in visceral adipose tissue glucose uptake
  • reduction in muscle lipotoxicity
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