Evaluate prebiotics using the high fat diet-fed mouse model

A dedicated nutritional animal model to demonstrate that your prebiotics impact metabolic parameters in adapted animal models i.e. early (4 weeks high fat feeding with glucose intolerance and impaired glucose-stimulated insulin secretion) or established (14 weeks high fat feeding with insulin resistance)

Key Benefits

► Validate a beneficial effects of your prebiotics on :

body weight and adipose tissue weight gain,
glucose intolerance and insulin resistance,
hepatic insulin signalling,
inflammation.

► Dedicated nutritional animal model to demonstrate that your compound improves both early (4 weeks high fat diet) or established (14 weeks high fat diet) diabetes.

Animal Model

Background Strain: C57Bl6/J mouse
Gender/Weight: Male
Diet: High Fat Diet
Time on diet: 4 weeks (impaired insulin secretion) or 14 weeks (impaired insulin action)

Scientific and pharmacological relevance

Mouse fed a control (chow-ctrl) or high fat diet (HF-ctrl) or high fat diet + fermentable diatary fiber oligofructose (HF- OFS) for 4 weeks (impaired insulin secretion) or 14 weeks (impaired insulin action).

Cani et al., Diabetes 55:1484-1490, 2006 ; Cani et al., Diabetologia 50: 2374-2383, 2007

Oligofructose decreases plasma glucose and increases plasma insulin levels (both fasted and fed conditions) in mice fed a 4-week high fat diet.
Oligofructose lowers body weight gain, epididymal fat pads and total energy intake in mice fed a 4-week high fat diet.

Oligofructose reduces glucose intolerance in diabetic mice (high fat diet for 4 weeks).
Oligofructose improves insulin sensitivity in diabetic mice fed a high fat diet for 4 weeks (increased glucose infusion rate and blunted hepatic glucose production both measured during an hyperinsulinemic euglycemic clamp).

Oligofructose improves hepatic insulin signaling by increasing phosphorylation of IRS and akt in mice fed a 4-week high fat diet.
Oligofructose reduces hepatic inflammation by decreasing phosphorylation of NF-Kb in mice fed a 4-week high fat diet.

Oligofructose decreases hyperinsulinemia in mice fed a high fat diet over 14 weeks.
Oligofructose reduces total body weight gain, epididymal fat pads and energy intake in mice fed a high fat diet over 14 weeks.

Oligofructose reduces glucose intolerance in diabetic mice (high fat diet over 14 weeks).
Oligofructose reduces inflammation in diabetic mice (high fat diet over 14 weeks).

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